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DsbA

Part:BBa_K243002

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-08)


DsbA signal sequence (enables periplasm export)

The signal sequence of the DsbA protein linked to the coding sequence of another protein enables export of the latter into the periplasm.


Usage and Biology

The DsbA gene encodes a periplasmic disulfide bond isomerase which posses a signal sequence. The protein is localized in the periplasm and catalyzes the formation of disulfide bonds. It therefore enables the correct folding of proteins in this compartment.
The signal sequence of the DsbA protein linked to the coding sequence of another protein enables export of the latter into the periplasm. The accumulation of the expressed protein in the periplasm can be used for the purification of the protein and to prevent interaction with cytoplasmic components. Freiburg 09 DsbA grafik.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None